RT @PatrickLemaire_
Oyez, Oyez, we are seeking to expand our team by recruiting 3 new colleagues, #PhDstudents and #postdoc. If you love #LiveImaging, #lightsheet, #scRNAseq, #smFISH, #QuantitativeBiology, #ascidians, #embryos and, please read on (and RT) 👇

#sciencejobs @SFbd @ISDB @TLM @SMBdevBio

This week's #FluorescenceFriday user is Jasmin Camacho, a postdoctoral research associate in the Rohner Lab at the Stowers Institute for Medical Research! Jasmin appreciates HCR RNA-FISH's ability to visualize multiple genes in a single sample, especially since she works with precious bat samples that are hard to acquire. Check out some of her stunning single molecule FISH images of the small intestinal villi and crypts of a nectar bat here:

#smFISH #HCRimaging #bat #genes #fluorescence

"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"

#mRNA #mRNALocalization #smFISH

https://www.nature.com/articles/s41596-022-00750-2

RS-FISH is a new #FIJI plugin for single-molecule FISH analysis
https://www.nature.com/articles/s41592-022-01669-y
I spent an hour playing with it. Installation was very easy & instructions of use are clear. I got nice spot detection on a not-so-nice FISH image.
I need to learn more about this tool, particularly using with masks & batch processing - but seems very nice & easy to use.
@preibischs @successprocess
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging

"RS-FISH: precise, interactive, fast, and scalable FISH spot detection"
New, user-friendly, FISH spot detection software; runs as a #Fiji plugin.
From Stephen Preibisch lab with @successprocess and Kyle Harrington
Out in @naturemethods
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging

https://www.nature.com/articles/s41592-022-01669-y