"Boosting the toolbox for live imaging of translation"

The authors developed a new set of #suntag tools (differnt green fluorescent proteins, different promoter for scFv) - optimizing them and identifying possible artefacts. these are primarily for #Drosophila but I guess could be adapted to other organisms.

They also developed a new system called ALFA_array which they test in fly and mammalian cells.

#mRNA #translation #microscopy #LiveCellImaging #SingleMolecule

https://www.biorxiv.org/content/10.1101/2023.02.25.529998v1