This #TechnicalTuesday we’re highlighting @haesleinhuepf 's post describing how to organise an international symposium. Robert organised the recent #PoLBIAS23 and shares his recommended timeline for organising a successful event.
https://focalplane.biologists.com/2023/09/05/a-schedule-for-organizing-international-symposia/

This #TechnicalTuesday is all about sample preparation with Joseph Frommelt, Fang Lin and colleagues. In @BiologyOpen, they present their novel deyolking method for whole-mount fluorescent imaging of zebrafish embryos.
https://journals.biologists.com/bio/article/12/7/bio060048/324207/Flat-mount-preparation-for-whole-mount-fluorescent

On #TechnicalTuesday we’re highlighting the @the_node post from @KuglerElisabeth, who shares a behind the scenes look at organising an art exhibition. Elisabeth’s exhibition opens in Nov 2023 & showcases the beauty of #devbio through #microscopy #sciart.
https://thenode.biologists.com/science-art-exhibition/science-art/

On #TechnicalTuesday we’re highlighting the paper from Vanessa Chong-Morrison, Tatjana Sauka-Spengler and colleagues on the use of Ac/Ds transposition for studying non-coding genomic elements in zebrafish embryos.
📖 @BiologyOpen
Article: https://journals.biologists.com/bio/article/12/6/bio059995/318441/Ac-Ds-transposition-for-CRISPR-dCas9-SID4x
Interview: https://journals.biologists.com/bio/article/12/6/bio060034/318462/First-person-Vanessa-Chong-Morrison

On #TechnicalTuesday we’re highlighting the OptIC-Notch tool from Jonathan Townson, Sarah Bray and colleagues based on the BLITz approach. The authors used this optogenetic method to temporally interrogate the interaction between Notch intracellular domain and the DNA-binding transcription factor CSL.

📖@Dev_journal: https://journals.biologists.com/dev/article/150/11/dev201785/316612/OptIC-Notch-reveals-mechanism-that-regulates

#microscopy #devbio

This #TechnicalTuesday we’re reading the #preLight from Girish Kale highlighting the beautiful #preprint on embryo mechanics from Sacha Ichbiah, Hervé Turlier, & team.

@preLights https://prelights.biologists.com/highlights/embryo-mechanics-cartography-inference-of-3d-force-atlases-from-fluorescence-microscopy/

This #TechnicalTuesday we are highlighting a FocalPlane post from Mara Lampert on tracking in napari. Mara describes the different tracking options, guides the user through the process & calls for feedback on the tools.
https://focalplane.biologists.com/2023/06/01/tracking-in-napari/
You can find Mara’s series, ‘Bio-image Analysis with Napari here: https://focalplane.biologists.com/2023/06/01/tracking-in-napari/

This #TechnicalTuesday we are highlighting zFACE, a tool to quantitatively assess craniofacial development in #zebrafish from Lorena Maili, George Eisenhoffer and colleagues.
Read the full #OpenAccess article in @DMM_Journal https://journals.biologists.com/dmm/article/16/6/dmm049868/313484/Facial-analytics-based-on-a-coordinate
First person interview: https://journals.biologists.com/dmm/article/16/6/dmm050302/313489/First-person-Lorena-Maili

On #TechnicalTuesday we’re highlighting the automated microscopy workflow from Arthur Lensen, Margarida C. Gomes, Ana Teresa López-Jiménez and Serge Mostowy. They used the workflow to study Shigella–neutrophil interactions and antibiotic efficacy in vivo.

📖@DMM_Journal:
https://journals.biologists.com/dmm/article/16/6/dmm049908/308879/An-automated-microscopy-workflow-to-study-Shigella

This #TechnicalTuesday we are highlighting tissue force microscopy (TiFM) from Chon Chan, Fengzhu Xiong, Arthur Michaut, Joana Vidigueira , Olivier Pourquié , L. Mahadevan. The authors use TiFM to measure forces in chick embryos with minimum invasiveness.

📖 @Dev_journal: https://journals.biologists.com/dev/article/150/9/dev201054/308160/Direct-force-measurement-and-loading-on-developing

This #TechnicalTuesday we are highlighting the correlative light and cryo-SXT pipeline used by Damián Lobato-Márquez @mostowylab and colleagues to dissect how septin-caged Shigella are targeted to the autophagic pathway.

📖 @J_Cell_Sci: https://journals.biologists.com/jcs/article/136/7/jcs261139/307062/Septins-and-K63-ubiquitin-chains-are-present-in

#OpenAccess #ReadandPublish

For #TechnicalTuesday we are looking at sharing data with @haesleinhuepf. Robert’s step-by-step guide explains how to make imaging data publicly available according FAIR principles on Zenodo.

https://focalplane.biologists.com/2023/02/15/sharing-research-data-with-zenodo/

It’s #TechnicalTuesday and we’re looking at segmentation. In their @BiologyOpen paper, @SaraRolfe and colleagues present MEMOS, a deep learning-enabled tool for segmentation of 3D imaging data from mouse embryos, which is implemented as an extension of the 3D Slicer App.
https://doi.org/10.1242/bio.059698

Find out more about Sara’s research in our ‘First person’ interview.
https://doi.org/10.1242/bio.059840
You can also check out their silver birch tree in the #forestofbiologists! https://forest.biologists.com/landscape/?id=85238

Size matters! This #TechnicalTuesday we’re highlighting Mara Lampert's post, ‘Rescaling mages and pixel (an)isotropy’. If you are acquiring 3D datasets, this is the post for you, as Mara takes us step-by-step through the rescaling required to account for anisotropic voxels between x (and y) and z and the impact failing to do this will have on your downstream analysis.

https://focalplane.biologists.com/2023/03/02/rescaling-images-and-pixel-anisotropy/

Solid as a rock! This #TechnicalTuesday we highlight Fast4DReg, a drift correction tool from Joanna Pylvänäinen, @guijacquemet and colleagues, developed for ImageJ or Fiji, which corrects axial and lateral drift, as well as misaligned channels.

@J_Cell_Sci: https://journals.biologists.com/jcs/article/136/4/jcs260728/287682/Fast4DReg-fast-registration-of-4D-microscopy

#microscopy #cellbiology #devbio

This #TechnicalTuesday we’re focussed on labelling. We look back at a #preLight from Martyna Kosno-Vega on a new method for tagging proteins for cryo-ET from Herman Fung, Yuki Hayashi, Veijo Salo, and team at EMBL Heidelberg.

https://prelights.biologists.com/highlights/genetically-encoded-multimeric-tags-for-intracellular-protein-localisation-in-cryo-em/
@preLights #preprint #microscopy

It’s #TechnicalTuesday, and this week we’re looking a DeepProjection from Daniel Haertter, Daniel Kiehart, Christoph Schmidt, et al., which extracts features from curved tissues imaged in 3D to project background free 2D images.

@Dev_journal: https://doi.org/10.1242/dev.200621

It's #TechnicalTuesday! Check out the #image analysis pipeline “GliaMorph” developed by
Elisabeth Kugler and team. This modular #analysis #toolkit provides a method to quantify complex glial #cell shape accurately and reproducibly.🔬

📖 @Dev_journal https://doi.org/10.1242/dev.201008