@PhiloNeuroScie Nice find! #Tissueclearing and #lightsheet #microscopy work on human brain (sections) too :)

Human brain samples are normally quite hard to properly image because they are dense with lipofuscins, super autofluorescent and not very permeable to antibodies. That they managed to pull this off using the more-or-less standard iDISCO protocol (even with "just" a 4 mm thick slab and high antibody concentrations) is quite impressive.

edits: corrected thickness + typos

Check out our lab's #newpaper in @cellpress Cell:
We found that the skull is special - its marrow is a special immunologic niche. Skull #Immune #cells can move to the meninges via skull-meninges connections, which we found both in mice and humans with #tissueclearing and #lightsheet #microscopy.
We also bring #proteomics and #PETimaging to the mix!

Paper: https://doi.org/10.1016/j.cell.2023.07.009

Explainer thread:
https://bird.makeup/users/erturklab/statuses/1689293936185991169

Preprint alert! Our lab developed targetable #DNA origami #nanoparticles, which we then image throughout the entire (transparent) mouse body using #lightsheet #microscopy.

Our DNA origami were able to target immune cells, as well as a subset of cancer metastases in the lung.

Preprint:
https://www.biorxiv.org/content/10.1101/2023.07.24.550304v1.full.pdf

Explainer thread:
https://bird.makeup/users/erturklab/statuses/1686331791022448640

#preprint #tissueclearing

New preprint by @elab : Check it out! We mapped SARS-CoV2 spike protein distribution in 3D throughout the mouse body, and at the human skull/brain interface.

We found spike protein accumulation in the skull-meninges-brain axis, lingering long after infection.

Preprint: https://www.biorxiv.org/content/10.1101/2023.04.04.535604v1.full.pdf

Explainer thread: https://twitter.com/erturklab/status/1643902019088908289

#covid #longcovid #lightsheet #tissueclearing #microscopy

More and more spatial proteomics techniques are out! This time DISCO-MS! Aided by AI and robotics, DISCO-MS generates proteome data indistinguishable from fresh samples. #proteomics #tissueclearing #deeplearning
https://www.cell.com/cell/fulltext/S0092-8674(22)01465-9