A self‐propagating, barcoded transposon system for the dynamic rewiring of genomic networks | Molecular Systems Biology
#tnseq
https://www.embopress.org/doi/full/10.15252/msb.202211398

#Genome-wide screen in human plasma identifies multifaceted complement evasion of #Pseudomonas aeruginosa | PLOS #Pathogens
#tnseq

https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011023

Quantifying the local #adaptive landscape of a nascent bacterial community

#tnseq #evolutionarypaths
https://www.nature.com/articles/s41467-022-35677-5

Revealing Causes for False-Positive and False-Negative Calling of Gene #Essentiality in Escherichia coli Using #Transposon Insertion Sequencing

#tnseq #functionalgenomics

https://journals.asm.org/doi/10.1128/msystems.00896-22

Construction of high-density #transposon mutant library of Staphylococcus aureus using #bacteriophage ϕ11 -
#tnseq

https://pubmed.ncbi.nlm.nih.gov/36422842/

So, I'm checking whether a transposon from a TB library for Tn-Seq experiments is actually present in the sequenced genome. #bioinformatics #microbiology #tuberculosis #tnseq Can I just try to align the transposon sequence to the contigs to see if its present? PCR failed to find the transposon in the isolate, so I should expect that the transposon sequence won't align to the contigs of the genome?