in #cancer cells, resistance can arise due to non-genetic differences

Emergent resistant colonies can represent various fate outcomes
--want to know where they came from

https://pubmed.ncbi.nlm.nih.gov/37468627/

single cell analysis reveals #transcriptional diversity in drug resistant fates, even within individual clones

but see "memory" in resistant states (drug treatment)

2/2

Loic Binan of the Broad:
All-optical genetic screens of intracellular and intercellular #transcriptional circuits with Perturb-FISH

Various CRISPR-based methods to look (multiplexed) at outcomes after knockdown to explore gene expression circuits, those are great, but want richer data (morphology etc)

one guide per cell (knock down one gene per cell) see #transcriptome via MERFISH, then identify the perturbed gene via in situ T7 transcription (same chemistry as MERFISH)

#CSSingleCells23

1/2

<checks calendar> Sunday?
🍅Perfect day to talk 🍅#tomatoes with Shi et al. who🍅review #transcriptional regulation of 🍅processes related to 🍅fleshy #fruit texture🔓⬇️🍅https://jipb.net/EN/10.1111/jipb.13316
@wileyplantsci
🍅 #PlantSci #development 🍅#CellWall #TF #MADSBoxProtein

“We further demonstrated #transcriptional changes in infected syncytiotrophoblasts that led to impairment of #cellular processes, reduced secretion of #HCG hormone and morphological changes vital for syncytiotrophoblast function. Furthermore, different #antibody strategies and #antiviral drugs restore these impairments.”

Shamless plug warning! ⚠️

Our last paper on #transcriptional heterogeneity of #pediatric #cancer is out.
Did you think a quiet #genome translates into a quiet #transcriptome? Think again!

#multiscale #clustering on a pan-cancer #RNASeq cohort revealed new diagnostic subtypes and helped us train a #cnn model to identify tumour components in #clinical #oncology samples.

Read it here, it's #openaccess: https://www.nature.com/articles/s41591-023-02221-x

#precisionmedicine #transcriptomics
#machinelearning #ml

PIF7-mediated #epigenetic reprogramming promotes the #transcriptional response to shade in #Arabidopsis

Chuanwei Yang et al.find when a plant grows in shade, transcription factor PIF7 triggers the adaptive growth response by guiding H3.3 deposition via the #histone chaperone #ASF1

https://www.embopress.org/doi/10.15252/embj.2022111472

RIViT-seq enables systematic identification of #regulons of #transcriptional machineries

https://www.nature.com/articles/s41467-022-31191-w

Water potential governs the effector specificity of the #transcriptional regulator XylR of #Pseudomonas putida -
#induction

https://ami-journals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.16342

#Transcriptional adaptation found to play a role in inherited #epigenetic changes.

#evolution

https://phys.org/news/2022-12-transcriptional-play-role-inherited-epigenetic.html

Insightful overview of where the field of blood biomarkers to diagnose infections is heading.

🎯 are all patients easily categorizable into bacterial or viral? (almost certainly 👎)
🎯 should we be aiming towards probability scores for different infection classes? (likely👍)
🎯will anything beat CRP or PCT in helping reduce unnecessary antibiotic prescriptions? 🤷

#transcriptional #biomarkers #viral #bacterial #blood #IDMastodon #MedMastodon
https://www.cell.com/cell-reports-medicine/fulltext/S2666-3791(22)00430-X?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS266637912200430X%3Fshowall%3Dtrue

Compendium-Wide #Analysis of #Pseudomonas #aeruginosa Core and Accessory Genes Reveals #Transcriptional Patterns across Strains #PAO1 and #PA14 | mSystems

https://journals.asm.org/doi/10.1128/msystems.00342-22

Species #interactions promote parallel #evolution of global #transcriptional regulators in a widespread #Staphylococcus species | bioRxiv
#microbialcommunities
https://www.biorxiv.org/content/10.1101/2022.12.19.521106v1

@edwardlau fantastic, #phosphoproteomics and #transcriptional profiling kinetics of #chromatin remodeling ?

https://www.omim.org/entry/100790

Here's my #introduction -
#Scientist and Associate Professor at the Department of Molecular and Cellular Physiology, Albany Medical College.
I research the mechanisms of #vascular #dysfunction leading to organ damage during severe #inflammation (e.g. #sepsis and septic #shock ) and its sequelae ( #kidney damage, #CognitiveDysfunction ). Working to find ways to prevent this by tackling the changes in the #endothelium. Interested in all things #signal #transduction and #transcriptional regulation