I have a question about DNA extractions of museum specimen for UCE capture. I am curious what the minimum input folks would feel comfortable using. I'm getting low amounts of DNA for a lot of old specimen 10-20 ng total (e.g., 50 uL @ 0.33 ng/uL). I might try sequencing low input samples on a separate plate, but would rather re-extract or extract new specimen.
#question
#ultraconservedelements
#museumspecimen
#phylogenetics
#systematics
#entomology