A new paper from @wu_xuebing 's lab just published @Nature
"Noncoding translation mitigation"

nature.com/articles/s41586-023

Here are my thoughts:
It is a very interesting paper, and important to anyone working on , particularly from , but not only.
In brief, they screened short sequences originating from or from non coding regions ( ) (and CDS as control) which they fused to GFP at the C-term and looked at GFP expression level.
>>

#mRNA #intron #utr #lncRNA #ncrnas #translation

Last updated 1 year ago

Ignite the Right · @UnmaskUTR
1612 followers · 119 posts · Server kolektiva.social

RT @SunlightAFA
Welcome to the Sunlight shithead Timothy Gallaher Murdock, aka "Horus the Avenger", from Dearborn Heights, Michigan twitter.com/UTRresearch/status

#utr #smithbaldutr

Last updated 1 year ago

Vikinkje · @vikinkje
5 followers · 72 posts · Server mastodon.nl

Wat kunnen bekerwedstrijden toch leuk zijn. -

#spa #utr

Last updated 1 year ago

Cosmin Saveanu · @cosminribo
45 followers · 105 posts · Server framapiaf.org

Seeing in cells, often involves the addition of MS2 binding sites in the 3' that can be detected with fluorescent coat protein. The MS2 binding site repeats increase the size of the 3' UTR and can also affect the degradation of fragments containing them: pubmed.ncbi.nlm.nih.gov/302181

Longer 3' UTR induce the degradation of the RNA of interest through nonsense-mediated mRNA decay (NMD). This can be avoided by tethering or a mutant .
biorxiv.org/content/10.1101/20

#PAB1 #eRF3 #rna #MS2 #utr #mRNAs

Last updated 2 years ago

Zic.it | Zeroincondott★ · @zic
933 followers · 4665 posts · Server mastodon.bida.im
Zic.it | Zeroincondott★ · @zic
933 followers · 4665 posts · Server mastodon.bida.im
Zic.it | Zeroincondott★ · @zic
933 followers · 4665 posts · Server mastodon.bida.im
Zic.it | Zeroincondott★ · @zic
933 followers · 4665 posts · Server mastodon.bida.im
Zic.it | Zeroincondott★ · @zic
933 followers · 4665 posts · Server mastodon.bida.im
Zic.it | Zeroincondott★ · @zic
933 followers · 4665 posts · Server mastodon.bida.im
Zic.it | Zeroincondott★ · @zic
933 followers · 4665 posts · Server mastodon.bida.im